Does anyone know where in this country you can get a blue LED torch with 47O nm wavelength? It must have a minimum of 5 LEDs
PS it's not intended for diving so doesn't have to be waterproof
The Technician in question wants this device to excite (oo-er missus!) fluorescent molecules, as many cnidarians (corals and anemones) have fluorescent properties, such a torch could be of interest of UW photographers
Its funny you should say this, how does a mercury arc with a 485 / 30 band pass filter sound. Tested last week, needs to be struck from the mains (trying to invent a way around this), but runs off 24V once lit.
Just need to finish housing off. Got a 50W bulb awith bout 200 hours life. Thought it would be fun to take pickies of fluorescence in the Red Sea in March. Can make another if you like. Blue LED's don't really have enough energy to get enough fluorescence for normal (non intergrating) CCD camera's.
Sounds pretty technical Andy (ie I've just ducked as that went over my head!).
Perhaps I should explain what the technician wants if for, she's working with transgenic mice which have the GFP gene inserted (I'm guessing you're familiar with that), a recent draft of a paper (Biotechniques, Vol 34 issue 2)) detailed a sytem where a commercially available blue LED flashlight (Inova X5 from Emissive Energy, USA) was used to illuminate the mice (through a Kodak Polymax PC3 filter), the mice are observed through another filter (Kodak Wratten Gelatin Filter 12). The whole idea is to emliminate the need to take a tissue sample (from the mouses ear) the paper acknowledges that the results aren't suitable for photomicrographs but is adequate for non-invasive identification of mice with the fluorescent gene.
Was in a shop over the weekend and was shown a torch utilising 3 white light LED's had a colour temperature of 6000 and an equivelant power output of 50W. All off three C cell batteries with a burn time of over two hours,